验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HaCaT cells, Jurkat cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32375-1-AP targets USP43 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36448 Product name: Recombinant human USP43 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 900-1123 aa of BC136368 Sequence: CLAPGNSDGPNTARKLKENAGQDIKLPRKFDLPLTVMPSVEHEKPARPEGQKAMNWKESFQMGSKSSPPSPYMGFSGNSKDSRRGTSELDRPLQGTLTLLRSVFRKKENRRNERAEVSPQVPPVSLVSGGLSPAMDGQAPGSPPALRIPEGLARGLGSRLERDVWSAPSSLRLPRKASRAPRGSALGMSQRTVPGEQASYGTFQRVKYHTLSLGRKKTLPESSF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 43 |
| 别名 | Deubiquitinating enzyme 43, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 43, Ubiquitin thioesterase 43, Ubiquitin-specific-processing protease 43 |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC136368 |
| 基因名称 | USP43 |
| Gene ID (NCBI) | 124739 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q70EL4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP43 belongs to the DUBs family involved in cancer development and progression.The USPs family is the most recognized group of DUBs, characterized by a wide range of structural and functional variability. The distinguishing features of USPs include the unique catalytic core, known as the histidine and cysteine boxes, as well as the presence of zinc finger domains, ubiquitin-binding domains, or ubiquitin-like domains at the N and/or Ctermini of the catalytic domain (PMID: 16325574). It has 4 isoforms with a molecular weight of 69 kDa, 89 kDa,122 kDa, 123 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP43 antibody 32375-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
