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验证数据展示

  • WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

    WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

    IP experiment of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

    IP result of anti-USP39 (IP:23865-1-AP, 4ug; Detection:23865-1-AP 1:1000) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

    WB analysis of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 23865-1-AP (same clone as 23865-1-PBS)

    WB analysis of HEK-293 using 23865-1-AP (same clone as 23865-1-PBS)

    WB result of USP39 antibody (23865-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP39 transfected HEK-293 cells. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

    WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

    IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 23865-1-AP (same clone as 23865-1-PBS)

    IF Staining of A431 using 23865-1-AP (same clone as 23865-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 23865-1-AP (same clone as 23865-1-PBS)

    IF Staining of A549 using 23865-1-AP (same clone as 23865-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 23865-1-AP (same clone as 23865-1-PBS)

    IF Staining of MCF-7 using 23865-1-AP (same clone as 23865-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP39 Polyclonal antibody, PBS Only

USP39 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 23865-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CGI 21, CGI21, CGI-21, EC:3.4.19.12, HSPC332

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

23865-1-PBS targets USP39 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20894

Product name: Recombinant human USP39 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-357 aa of BC067273

Sequence: MSGRSKRESRGSTRGKRESESRGSSGRVKRERDREREPEAASSRGSPVRVKREFEPASAREAPASVVPFVRVKREREVDEDSEPEREVRAKNGRVDSEDRRSRHCPYLDTINRSVLDFDFEKLCSISLSHINAYACLVCGKYFQGRGLKSHAYIHSVQFSHHVFLNLHTLKFYCLPDNYEIIDSSLEDITYVLKPTFTKQQIANLDKQAKLSRAYDGTTYLPGIVGLNNIKANDYANAVLQALSNVPPLRNYFLEEDNYKNIKRPPGDIMFLLVQRFGELMRKLWNPRNFKAHVSPHEMLQAVVLCSKKTFQITKQGDGVDFLSWFLNALHSALGGTKKKKKTIVTDVFQGSMRIFT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 39
别名 CGI 21, CGI21, CGI-21, EC:3.4.19.12, HSPC332
计算分子量 565 aa, 65 kDa
观测分子量 60~65 kDa
GenBank蛋白编号BC067273
基因名称 USP39
Gene ID (NCBI) 10713
RRIDAB_2879343
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53GS9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP39 plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development. USP39 regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2

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