验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells |
| Positive IP detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy USP38 Ready-To-Use IHC Kit. USP38 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17767-1-AP targets USP38 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | USP38 fusion protein Ag12172 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 38 |
| 别名 | Ubiquitin thiolesterase 38, Ubiquitin carboxyl-terminal hydrolase 38, KIAA1891, HP43.8KD, EC:3.4.19.12 |
| 计算分子量 | 1042 aa, 117 kDa |
| 观测分子量 | 117 kDa |
| GenBank蛋白编号 | BC068975 |
| 基因名称 | USP38 |
| Gene ID (NCBI) | 84640 |
| RRID | AB_2213383 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NB14 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin specific peptidase 38 (USP38) is a negative regulator of type I IFN signaling. USP38 can strongly induced the degradation of the active form of TBK1 after viral infection. USP38 may play a critical role in the maintenance of the balance between innate immune responses and tolerance through dynamic ubiquitin editing of TBK1 in response to viral infection and perhaps other infectious diseases or cancer. (PMID:27692986)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP38 antibody 17767-1-AP | Download protocol |
| IP protocol for USP38 antibody 17767-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Med USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.
| ||
Oncogenesis USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.
| ||
Am J Cancer Res Ubiquitin specific peptidase 38 promotes the progression of gastric cancer through upregulation of fatty acid synthase.
| ||
J Biol Chem ADAR promotes USP38 auto-deubiquitylation and stabilization in an RNA editing-independent manner in Esophageal Squamous Cell Carcinoma | ||
Int J Biochem Cell Biol The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc. | ||
Mol Med USP38 exacerbates pressure overload-induced left ventricular electrical remodeling |