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验证数据展示

  • IHC staining of human breast cancer using 18827-1-AP

    IHC staining of human breast cancer using 18827-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18827-1-AP (USP34 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 18827-1-AP

    IHC staining of human breast cancer using 18827-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18827-1-AP (USP34 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP34 Polyclonal antibody

USP34 Polyclonal Antibody for IHC, ELISA
Cat No. 18827-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

Deubiquitinating enzyme 34, KIAA0570, KIAA0729, Ubiquitin thiolesterase 34, USP34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18827-1-AP targets USP34 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 34
别名 Deubiquitinating enzyme 34, KIAA0570, KIAA0729, Ubiquitin thiolesterase 34, USP34
计算分子量 404 kDa
GenBank蛋白编号BC022783
基因名称 USP34
Gene ID (NCBI) 9736
RRIDAB_2213348
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70CQ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP34, also named as KIAA0570 and KIAA0729, belongs to the peptidase C19 family. USP34 recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. It is involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. The antibody recognize all these 3 isoforms of USP34.

实验方案

Product Specific Protocols
IHC protocol for USP34 antibody 18827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols