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验证数据展示

  • WB analysis of HEK-293 using 12060-2-AP

    WB analysis of HEK-293 using 12060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12060-2-AP (USP33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 12060-2-AP

    IHC staining of human medulloblastoma using 12060-2-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12060-2-AP (USP33 antibody) at dilution of 1:50 (under 10x lens).

USP33 Polyclonal antibody

USP33 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12060-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Ubiquitin-specific-processing protease 33, Ubiquitin thioesterase 33, Ubiquitin carboxyl-terminal hydrolase 33, KIAA1097, hVDU1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman medulloblastoma tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12060-2-AP targets USP33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2695

Product name: Recombinant human USP33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC016663

Sequence: MTGSNSHITILTLKVLPHFESLGKQEKIPNKMSAFRNHCPHLDSVGEITKEDLIQKSLGTCQDCKVQGPNLWACLENRCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLRVWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFKIPSNTTLKTPLVAVFDDLDIEADEEDELRARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEVEEDPQT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 33
别名 Ubiquitin-specific-processing protease 33, Ubiquitin thioesterase 33, Ubiquitin carboxyl-terminal hydrolase 33, KIAA1097, hVDU1
计算分子量 942 aa, 107 kDa
观测分子量 107 kDa/ 103 kDa/ 93 kDa
GenBank蛋白编号BC016663
基因名称 USP33
Gene ID (NCBI) 23032
RRIDAB_2213235
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEY7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for USP33 antibody 12060-2-APDownload protocol
IHC protocol for USP33 antibody 12060-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols