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验证数据展示

  • WB analysis using 18838-1-AP

    WB analysis using 18838-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18838-1-AP (USP32 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 18838-1-AP

    IHC staining of human pancreas using 18838-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18838-1-AP (USP32 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas using 18838-1-AP

    IHC staining of human pancreas using 18838-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 18838-1-AP (USP32 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of mouse testis using 18838-1-AP

    IHC staining of mouse testis using 18838-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18838-1-AP (USP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

USP32 Polyclonal antibody

USP32 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18838-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

USP10, Ubiquitin-specific-processing protease 32, Ubiquitin carboxyl-terminal hydrolase 32, Renal carcinoma antigen NY-REN-60, NY REN 60

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inmouse testis tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18838-1-AP targets USP32 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human, mouse
文献引用反应性human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 32
别名 USP10, Ubiquitin-specific-processing protease 32, Ubiquitin carboxyl-terminal hydrolase 32, Renal carcinoma antigen NY-REN-60, NY REN 60
计算分子量 182 kDa
观测分子量230 kDa
GenBank蛋白编号BC094680
基因名称 USP32
Gene ID (NCBI) 84669
RRIDAB_10598326
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NFA0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP32 belongs to the peptidase C19 family. USP32 catalytic the reaction :Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

实验方案

Product Specific Protocols
WB protocol for USP32 antibody 18838-1-APDownload protocol
IHC protocol for USP32 antibody 18838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Basis Dis

USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis

Authors - Shun Gao
View Article
humanWB,CoIP

Acta Biochim Biophys Sin (Shanghai)

Roles of the hsa_circ_0013880/USP32/Rap1b axis in the proliferation and apoptosis of acute myeloid leukemia cells

Authors - Heyang Zhang
  • KD Validated
View Article