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验证数据展示

  • WB analysis using 32265-1-AP

    WB analysis using 32265-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32265-1-AP (USP31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

USP31 Polyclonal antibody

USP31 Polyclonal Antibody for WB, ELISA
Cat No. 32265-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Ubiquitin thioesterase 31, KIAA1203, Deubiquitinating enzyme 31

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHaCaT cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32265-1-AP targets USP31 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 USP31 fusion protein Ag36315 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 31
别名 Ubiquitin thioesterase 31, KIAA1203, Deubiquitinating enzyme 31
计算分子量146kDa
观测分子量147 kDa, 47 kDa
GenBank蛋白编号NM_020718
基因名称 USP31
Gene ID (NCBI) 57478
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ70CQ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP31 (Ubiquitin Specific Peptidase 31) is a protein-coding gene that encodes a thiol-dependent deubiquitinating enzyme. It is involved in the process of protein deubiquitination, which is essential for regulating protein stability, function, and degradation. USP31 is broadly expressed in various tissues, including the testis and urinary bladder. It has 2 isoforms with the molecular mass of 47 and 147 kDa.

实验方案

Product Specific Protocols
WB protocol for USP31 antibody 32265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols