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验证数据展示

  • WB analysis using 27522-1-AP

    WB analysis using 27522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27522-1-AP (USP29 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 27522-1-AP

    IHC staining of mouse testis using 27522-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27522-1-AP (USP29 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27522-1-AP

    IHC staining of mouse testis using 27522-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27522-1-AP (USP29 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

USP29 Polyclonal antibody

USP29 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27522-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat and More (1)

应用

WB, IHC, ELISA and More (1)

别名

Deubiquitinating enzyme 29, HOM TES 84/86, Ubiquitin thiolesterase 29, USP29

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27522-1-AP targets USP29 in WB, IHC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 Human, Mouse, Rat
文献引用反应性mouse
免疫原 USP29 fusion protein Ag23231 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 29
别名 Deubiquitinating enzyme 29, HOM TES 84/86, Ubiquitin thiolesterase 29, USP29
观测分子量 104 kDa
GenBank蛋白编号BC130394
基因名称 USP29
Gene ID (NCBI) 57663
RRIDAB_2880898
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for USP29 antibody 27522-1-APDownload protocol
IHC protocol for USP29 antibody 27522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Ubiquitin-Specific Protease 29 Exacerbates Cerebral Ischemia-Reperfusion Injury in Mice.

Authors - Jia-Bao Hou
  • KO Validated
View Article
mouseWB,IP

Redox Biol

ALDH2 deficiency augments atherosclerosis through the USP14-cGAS-dependent polarization of proinflammatory macrophages

Authors - Haiying Rui
View Article
mouseIHC

Clin Mol Hepatol

USP29 alleviates the progression of MASLD by stabilizing ACSL5 through K48 deubiquitination

Authors - Sha Hu
View Article