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验证数据展示

  • WB analysis using 67814-1-Ig (same clone as 67814-1-PBS)

    WB analysis using 67814-1-Ig (same clone as 67814-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67814-1-Ig (USP28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

  • WB analysis using 67814-1-Ig (same clone as 67814-1-PBS)

    WB analysis using 67814-1-Ig (same clone as 67814-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67814-1-Ig (USP28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67814-1-Ig (same clone as 67814-1-PBS)

    IF Staining of HeLa using 67814-1-Ig (same clone as 67814-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using USP28 antibody (67814-1-Ig, Clone: 1E3D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67814-1-PBS in a different storage buffer formulation.

USP28 Monoclonal antibody, PBS Only

USP28 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67814-1-PBS

产品说明书

CloneNo. 1E3D1

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

1E3D1, Deubiquitinating enzyme 28, EC:3.4.19.12, KIAA1515, Ubiquitin carboxyl-terminal hydrolase 28

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67814-1-PBS targets USP28 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11805

Product name: Recombinant human USP28 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC065928

Sequence: MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKY

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 28
别名 1E3D1, Deubiquitinating enzyme 28, EC:3.4.19.12, KIAA1515, Ubiquitin carboxyl-terminal hydrolase 28
计算分子量583aa,66 kDa; 1077aa,120 kDa
观测分子量 135 kDa
GenBank蛋白编号BC065928
基因名称 USP28
Gene ID (NCBI) 57646
RRIDAB_2918577
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96RU2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP28 (Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).

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