验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, mouse heart tissue, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17707-1-AP targets USP28 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12003 Product name: Recombinant human USP28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC065928 Sequence: MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 28 |
| 别名 | Deubiquitinating enzyme 28, EC:3.4.19.12, KIAA1515, Ubiquitin carboxyl-terminal hydrolase 28, Ubiquitin thiolesterase 28 |
| 计算分子量 | 583aa,66 kDa; 1077aa,120 kDa |
| 观测分子量 | 135 kDa |
| GenBank蛋白编号 | BC065928 |
| 基因名称 | USP28 |
| Gene ID (NCBI) | 57646 |
| RRID | AB_2272676 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96RU2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP28(Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for USP28 antibody 17707-1-AP | Download protocol |
| WB protocol for USP28 antibody 17707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cancer The deubiquitinase USP25 supports colonic inflammation and bacterial infection and promotes colorectal cancer. | ||
Nat Commun USP5 stabilizes YTHDF1 to control cancer immune surveillance through mTORC1-mediated phosphorylation | ||
Mol Cell Distinct USP25 and USP28 Oligomerization States Regulate Deubiquitinating Activity. | ||
Nucleic Acids Res The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage | ||
J Clin Invest The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.
| ||
Adv Sci (Weinh) PFKFB4 Deubiquitination by USP10 Enhances Fumarate Metabolism to Orchestrate the KDM1A/Rad51 Axis and Confer Radioresistance in Lung Cancer. |
