验证数据展示
经过测试的应用
| Positive WB detected in | A-673 cells, BxPC-3 cells, J774A.1 cells, mouse cerebellum tissue, HEK-293 cells, K-562 cells, RAW 264.7 cells |
| Positive IP detected in | RAW 264.7 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12199-1-AP targets USP25 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2837 Product name: Recombinant human USP25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 171-451 aa of BC015930 Sequence: LKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 25 |
| 别名 | Deubiquitinating enzyme 25, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 25, Ubiquitin thioesterase 25, Ubiquitin thiolesterase 25 |
| 计算分子量 | 125 kDa |
| 观测分子量 | 125 kDa |
| GenBank蛋白编号 | BC015930 |
| 基因名称 | USP25 |
| Gene ID (NCBI) | 29761 |
| RRID | AB_2212771 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UHP3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP25 antibody 12199-1-AP | Download protocol |
| IP protocol for USP25 antibody 12199-1-AP | Download protocol |
| WB protocol for USP25 antibody 12199-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage | ||
Adv Sci (Weinh) USP25 Elevates SHLD2-Mediated DNA Double-Strand Break Repair and Regulates Chemoresponse in Cancer | ||
Adv Sci (Weinh) PFKFB4 Deubiquitination by USP10 Enhances Fumarate Metabolism to Orchestrate the KDM1A/Rad51 Axis and Confer Radioresistance in Lung Cancer. | ||
Cell Death Dis TRMT1-mediated tRNA m(2)(2)G modification drives Osimertinib resistance via the ATXN3/USP25 axis in lung adenocarcinoma. | ||
Cell Death Dis Targeting Irgm1 to combat osteoporosis: suppressing ROS and restoring bone remodeling |
