验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse lung tissue, rat lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lymphoma tissue, rat stomach tissue, human placenta tissue, rat pancreas tissue, mouse liver tissue, mouse stomach tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy® USP24 Ready-To-Use IHC Kit. USP24 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13126-1-AP targets USP24 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3779 Product name: Recombinant human USP24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2312-2620 aa of BC029660 Sequence: MISFLLGASRQNNQIRRWSSAQAREFGNLHNTVALLVLHSDVSSQRNVAPGIFKQRPPISIAPSSPLLPLHEEVEALLFMSEGKPYLLEVMFALRELTGSLLALIEMVVYCCFCNEHFSFTMLHFIKNQLETAPPHELKNTFQLLHEILVIEDPIQVERVKFVFETENGLLALMHHSNHVDSSRCYQCVKFLVTLAQKCPAAKEYFKENSHHWSWAVQWLQKKMSEHYWTPQSNVSNETSTGKTFQRTISAQDTLAYATALLNEKEQSGSSNGSESSPANENGDRHLQQGSESPMMIGELRSDLDDVDP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 24 |
| 别名 | Deubiquitinating enzyme 24, EC:3.4.19.12, KIAA1057, Ubiquitin carboxyl-terminal hydrolase 24, Ubiquitin thioesterase 24 |
| 计算分子量 | 294 kDa |
| 观测分子量 | 280-294 kDa |
| GenBank蛋白编号 | BC029660 |
| 基因名称 | USP24 |
| Gene ID (NCBI) | 23358 |
| RRID | AB_2212761 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UPU5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for USP24 antibody 13126-1-AP | Download protocol |
| IHC protocol for USP24 antibody 13126-1-AP | Download protocol |
| IP protocol for USP24 antibody 13126-1-AP | Download protocol |
| WB protocol for USP24 antibody 13126-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma | ||
Mol Cancer LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer | ||
Autophagy The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability.
| ||
Nat Commun ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. | ||
Nat Commun A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.
| ||
Nat Commun USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.
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