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- KD/KO Validated
USP2 Polyclonal antibody
USP2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells, mouse brain tissue, mouse kidney tissue, MCF-7 cells, HEK-293 cells |
Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse embryo tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10392-1-AP targets USP2 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | USP2 fusion protein Ag0591 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ubiquitin specific peptidase 2 |
Synonyms | Deubiquitinating enzyme 2, ubiquitin specific peptidase 2, Ubiquitin thiolesterase 2, UBP41, USP2, USP9 |
Calculated Molecular Weight | 41 kDa, 69 kDa |
Observed Molecular Weight | 68 kDa, 41-45 kDa |
GenBank Accession Number | BC002955 |
Gene Symbol | USP2 |
Gene ID (NCBI) | 9099 |
RRID | AB_2212432 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O75604 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for USP2 antibody 10392-1-AP | Download protocol |
IHC protocol for USP2 antibody 10392-1-AP | Download protocol |
IF protocol for USP2 antibody 10392-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
FASEB J Ubiquitin-specific protease 2-69 in macrophages potentially modulates metainflammation. | ||
J Cell Mol Med Ubiquitin-specific protease 2 regulates Ang Ⅱ-induced cardiac fibroblasts activation by up-regulating cyclin D1 and stabilizing β-catenin in vitro.
| ||
Mol Nutr Food Res 3,3'-Diindolylmethane suppresses high-fat diet-induced obesity through inhibiting adipogenesis of pre-adipocytes by targeting USP2 activity. | ||
Mediators Inflamm Overexpression of Ubiquitin-Specific Protease 2 (USP2) in the Heart Suppressed Pressure Overload-Induced Cardiac Remodeling. | ||
J Cancer Res Clin Oncol USP2 promotes cell proliferation and metastasis in choroidal melanoma via stabilizing Snail | ||
Tumour Biol USP2 promotes cell migration and invasion in triple negative breast cancer cell lines. |