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验证数据展示

  • WB analysis of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

    WB analysis of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

    IP experiment of Jurkat using 25768-1-AP (same clone as 25768-1-PBS)

    IP result of anti-USP19 (IP:25768-1-AP, 4ug; Detection:25768-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    WB analysis using 25768-1-AP (same clone as 25768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 25768-1-AP (same clone as 25768-1-PBS)

    WB analysis of HeLa using 25768-1-AP (same clone as 25768-1-PBS)

    WB result of USP19 antibody (25768-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP19 transfected HeLa cells. This data was developed using the same antibody clone with 25768-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

USP19 Polyclonal antibody, PBS Only

USP19 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 25768-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

EC:3.4.19.12, KIAA0891, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25768-1-PBS targets USP19 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22646

Product name: Recombinant human USP19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 203-500 aa of BC146752

Sequence: VEADEQLCIPPLNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVRSRNPGKDDCAKEEMAVAADAATLVDEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEICRDTSRVLFREQDFTLIFQTRDGNFLRLHPGCGPHTTFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPAARVGGAKVAVPTGPTPLDSTPPGGAPHPLTGQEEARAVEKDKSKARSEDTGLDSVATRTPMEHVTPKPETHLASPKPTCMVPPMPHSPVSGDSVEEEEEEEKKVCLPGFTGLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 19
别名 EC:3.4.19.12, KIAA0891, Ubiquitin carboxyl-terminal hydrolase 19, Ubiquitin thioesterase 19, Ubiquitin-specific-processing protease 19
计算分子量 1318 aa, 146 kDa
观测分子量 70 kDa, 120 kDa, 150-180 kDa
GenBank蛋白编号BC146752
基因名称 USP19
Gene ID (NCBI) 10869
RRIDAB_2713918
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94966
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).

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