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验证数据展示

  • WB analysis of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

    WB analysis of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

    WB result of USP18 antibody(12153-1-AP) with MCF-7 cells (Control and CRISPR USP18). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 12153-1-AP (same clone as 12153-1-PBS)

    IHC staining of human colon cancer using 12153-1-AP (same clone as 12153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12153-1-AP (USP18 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

    IF Staining of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP18 antibody (12153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

USP18 Polyclonal antibody, PBS Only

USP18 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12153-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

43 kDa ISG15-specific protease, EC:3.4.19.12, hUBP43, ISG15-specific-processing protease, ISG43

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12153-1-PBS targets USP18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2785

Product name: Recombinant human USP18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-372 aa of BC014896

Sequence: MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNIGQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRPLELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVDCAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGKQVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGGQYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQETAYLLVYMKMEC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 18
别名 43 kDa ISG15-specific protease, EC:3.4.19.12, hUBP43, ISG15-specific-processing protease, ISG43
计算分子量 372 aa, 43 kDa
观测分子量 43 kDa, 70 kDa
GenBank蛋白编号BC014896
基因名称 USP18
Gene ID (NCBI) 11274
RRIDAB_2877830
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMW8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP18(UBP43) is a 43 kDa protein that is known as an ISG15-deconjugating enzyme and an inhibitor of type I IFN signaling independent of its enzyme activity. USP18 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. It can be detected two bands(apparent MW of 38 and 35 kDa), the faster migrating one results from proteolytic processing in human primary hepatocytes(PMID:21779393). Sometimes the 70 kDa band is showed on the SDS-PAGE that it is considered as the complex of ISG15 and USP18(PMID: 14673145).

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