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验证数据展示

  • WB analysis using 82128-1-RR

    WB analysis using 82128-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82128-1-RR

    WB analysis of HeLa using 82128-1-RR

    WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells.

  • IP experiment of mouse liver using 82128-1-RR

    IP experiment of mouse liver using 82128-1-RR

    IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug.

  • Affinity and Kinetic Characterization of 82128-1-RR

    Affinity and Kinetic Characterization of 82128-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

USP14 Recombinant antibody

USP14 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 82128-1-RR

产品说明书

CloneNo. 3G12

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IP, ELISA

别名

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HEK-293T cells, DU 145 cells, Jurkat cells, NIH/3T3 cells, C6 cells
Positive IP detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82128-1-RR targets USP14 in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 USP14 fusion protein Ag5979 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
别名 Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14
计算分子量 54 kDa
观测分子量60 kDa
GenBank蛋白编号BC003556
基因名称 USP14
Gene ID (NCBI) 9097
RRIDAB_3086466
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54578
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

实验方案

Product Specific Protocols
WB protocol for USP14 antibody 82128-1-RRDownload protocol
IP protocol for USP14 antibody 82128-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols