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USP14 Recombinant antibody

USP14 Recombinant Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G12

Cat No : 82128-1-RR

Print datasheet

Synonyms

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

验证数据展示

  • WB analysis using 82128-1-RR

    WB analysis using 82128-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82128-1-RR

    WB analysis of HeLa using 82128-1-RR

    WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells.

  • IP experiment of mouse liver using 82128-1-RR

    IP experiment of mouse liver using 82128-1-RR

    IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug.

经过测试的应用

Positive WB detected inHCT 116 cells, HeLa cells, HEK-293T cells, DU 145 cells, Jurkat cells, NIH/3T3 cells, C6 cells
Positive IP detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82128-1-RR targets USP14 in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen USP14 fusion protein Ag5979 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Synonyms Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14
Calculated Molecular Weight 54 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC003556
Gene Symbol USP14
Gene ID (NCBI) 9097
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54578
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

实验方案

Product Specific Protocols
WB protocol for USP14 antibody 82128-1-RRDownload protocol
IP protocol for USP14 antibody 82128-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols