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USP11 Polyclonal antibody

USP11 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10244-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, IHC, ELISA

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, human testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy USP11 Ready-To-Use IHC Kit. USP11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10244-1-AP targets USP11 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 USP11 fusion protein Ag0296 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 11
别名 Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11
计算分子量 963 aa, 110 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC000350
基因名称 USP11
Gene ID (NCBI) 8237
RRIDAB_2288400
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51784
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

实验方案

Product Specific Protocols
WB protocol for USP11 antibody 10244-1-APDownload protocol
IHC protocol for USP11 antibody 10244-1-APDownload protocol
IP protocol for USP11 antibody 10244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair.

Authors - Marek Adamowicz
humanWB

Theranostics

A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.

Authors - Qi Zhang
humanWB,IHC

Clin Transl Med

EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.

Authors - Hongyu Zhao
humanIHC

Oncogene

The deubiquitinase USP11 regulates cell proliferation and ferroptotic cell death via stabilization of NRF2 USP11 deubiquitinates and stabilizes NRF2.

Authors - Chunjie Meng
humanWB,IHC

Cancer Lett

The E2F1/USP11 positive feedback loop promotes hepatocellular carcinoma metastasis and inhibits autophagy by activating ERK/mTOR pathway.

Authors - Lijun Qiao
  • KD Validated
human,mouseWB

Int J Radiat Oncol Biol Phys

USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling

Authors - Yiting Tang
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