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验证数据展示

  • WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of NIH/3T3 using 66069-1-Ig (same clone as 66069-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of HSC-T6 using 66069-1-Ig (same clone as 66069-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP1 Monoclonal antibody, PBS Only

USP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66069-1-PBS

产品说明书

CloneNo. 1C10H6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

1C10H6, Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66069-1-PBS targets USP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5904

Product name: Recombinant human USP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 437-785 aa of BC050525

Sequence: RTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 1
别名 1C10H6, Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1
计算分子量 88 kDa
观测分子量 100 kDa
GenBank蛋白编号BC050525
基因名称 USP1
Gene ID (NCBI) 7398
RRIDAB_11043340
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94782
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).

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