验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14346-1-AP targets USP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5686 Product name: Recombinant human USP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 437-785 aa of BC050525 Sequence: RTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 1 |
| 别名 | Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1, Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment |
| 计算分子量 | 88 kDa |
| 观测分子量 | 110 kDa, 100 kDa |
| GenBank蛋白编号 | BC050525 |
| 基因名称 | USP1 |
| Gene ID (NCBI) | 7398 |
| RRID | AB_2214314 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94782 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. It is also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases (PMID: 9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment (PMID: 18082604).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for USP1 antibody 14346-1-AP | Download protocol |
| IP protocol for USP1 antibody 14346-1-AP | Download protocol |
| WB protocol for USP1 antibody 14346-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Chem Biol A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. | ||
Mol Cell Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress. | ||
Nat Commun The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites. | ||
Nat Commun hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma. | ||
Theranostics CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells |
