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USH1G Polyclonal antibody

USH1G Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat No : 21936-1-AP

Print datasheet

Synonyms

ANKS4A, SANS, USH1G, Usher syndrome type 1G protein

验证数据展示

  • IHC staining of mouse testis using 21936-1-AP

    IHC staining of mouse testis using 21936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USH1G antibody included
  • IHC staining of mouse testis using 21936-1-AP

    IHC staining of mouse testis using 21936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USH1G antibody included
  • IP experiment of NIH/3T3 using 21936-1-AP

    IP experiment of NIH/3T3 using 21936-1-AP

    IP result of anti-USH1G (IP:21936-1-AP, 4ug; Detection:21936-1-AP 1:500) with NIH/3T3 cells lysate 1200ug.

经过测试的应用

Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy USH1G Ready-To-Use IHC Kit. USH1G primary antibody included.

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21936-1-AP is USH1G Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, ELISA
Tested Reactivity Mouse
Immunogen USH1G fusion protein Ag16571 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Usher syndrome 1G (autosomal recessive)
Synonyms ANKS4A, SANS, USH1G, Usher syndrome type 1G protein
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC101099
Gene Symbol USH1G
Gene ID (NCBI) 124590
RRIDAB_2878950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ495M9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for USH1G antibody 21936-1-APDownload protocol
IP protocol for USH1G antibody 21936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols