验证数据展示
经过测试的应用
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy USH1G Ready-To-Use IHC Kit. USH1G primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21936-1-AP targets USH1G in WB, IHC, IF, IP, ELISA applications and shows reactivity with Mouse samples.
| 经测试应用 | IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Mouse |
| 文献引用反应性 | human |
| 免疫原 | USH1G fusion protein Ag16571 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Usher syndrome 1G (autosomal recessive) |
| 别名 | ANKS4A, SANS, USH1G, Usher syndrome type 1G protein |
| 计算分子量 | 461 aa, 51 kDa |
| 观测分子量 | 52-55 kDa |
| GenBank蛋白编号 | BC101099 |
| 基因名称 | USH1G |
| Gene ID (NCBI) | 124590 |
| RRID | AB_2878950 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q495M9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for USH1G antibody 21936-1-AP | Download protocol |
| IP protocol for USH1G antibody 21936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
