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验证数据展示

  • IHC staining of mouse testis using 21936-1-AP

    IHC staining of mouse testis using 21936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USH1G antibody included
  • IHC staining of mouse testis using 21936-1-AP

    IHC staining of mouse testis using 21936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21936-1-AP (USH1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USH1G antibody included
  • IP experiment of NIH/3T3 using 21936-1-AP

    IP experiment of NIH/3T3 using 21936-1-AP

    IP result of anti-USH1G (IP:21936-1-AP, 4ug; Detection:21936-1-AP 1:500) with NIH/3T3 cells lysate 1200ug.

USH1G Polyclonal antibody

USH1G Polyclonal Antibody for IP, IHC, ELISA
Cat No. 21936-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Mouse and More (1)

应用

IP, IHC, ELISA and More (2)

别名

ANKS4A, SANS, USH1G, Usher syndrome type 1G protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy USH1G Ready-To-Use IHC Kit. USH1G primary antibody included.

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21936-1-AP targets USH1G in WB, IHC, IF, IP, ELISA applications and shows reactivity with Mouse samples.

经测试应用 IP, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Mouse
文献引用反应性human
免疫原

CatNo: Ag16571

Product name: Recombinant human USH1G protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 81-411 aa of BC101099

Sequence: LSFLVSFGANIWCLDNDYHTPLDMAAMKGHMECVRYLDSIAAKQSSLNPKLVGKLKDKAFREAERRIRECAKLQRRHHERMERRYRRELAERSDTLSFSSLTSSTLSRRLQHLALGSHLPYSQATLHGTARGKTKMQKKLERRKQGGEGTFKVSEDGRKSARSLSGLQLGSDVMFVRQGTYANPKEWGRAPLRDMFLSDEDSVSRATLAAEPAHSEVSTDSGHDSLFTRPGLGTMVFRRNYLSSGLHGLGREDGGLDGVGAPRGRLQSSPSLDDDSLGSANSLQDRSCGEELPWDELDLGLDEDLEPETSPLETFLASLHMEDFAALLRQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Usher syndrome 1G (autosomal recessive)
别名 ANKS4A, SANS, USH1G, Usher syndrome type 1G protein
计算分子量 461 aa, 51 kDa
观测分子量 52-55 kDa
GenBank蛋白编号BC101099
基因名称 USH1G
Gene ID (NCBI) 124590
RRIDAB_2878950
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ495M9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for USH1G antibody 21936-1-APDownload protocol
IP protocol for USH1G antibody 21936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cells

Nuclear-Cytoplasmic Shuttling of the Usher Syndrome 1G Protein SANS Differs from Its Paralog ANKS4B

Authors - Jacques S Fritze
  • KD Validated
View Article