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UROS Polyclonal antibody

UROS Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14913-1-AP

Print datasheet

Synonyms

UROIIIS, uroporphyrinogen III synthase, UROS

验证数据展示

  • WB analysis using 14913-1-AP

    WB analysis using 14913-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 14913-1-AP

    IP experiment of K-562 using 14913-1-AP

    IP result of anti-UROS (IP:14913-1-AP, 4ug; Detection:14913-1-AP 1:500) with K-562 cells lysate 4000ug.

  • IF Staining of MCF-7 using 14913-1-AP

    IF Staining of MCF-7 using 14913-1-AP

    Immunofluorescent analysis of MCF-7 cells, using UROS antibody 14913-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human cervical cancer using 14913-1-AP

    IHC staining of human cervical cancer using 14913-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 14913-1-AP

    IHC staining of human cervical cancer using 14913-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14913-1-AP targets UROS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen UROS fusion protein Ag6711 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uroporphyrinogen III synthase
Synonyms UROIIIS, uroporphyrinogen III synthase, UROS
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC002573
Gene Symbol UROS
Gene ID (NCBI) 7390
RRIDAB_2213824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10746
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UROS antibody 14913-1-APDownload protocol
IHC protocol for UROS antibody 14913-1-APDownload protocol
IF protocol for UROS antibody 14913-1-APDownload protocol
IP protocol for UROS antibody 14913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols