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验证数据展示

  • WB analysis using 14913-1-AP

    WB analysis using 14913-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14913-1-AP (UROS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 14913-1-AP

    IP experiment of K-562 using 14913-1-AP

    IP result of anti-UROS (IP:14913-1-AP, 4ug; Detection:14913-1-AP 1:500) with K-562 cells lysate 4000ug.

  • IF Staining of MCF-7 using 14913-1-AP

    IF Staining of MCF-7 using 14913-1-AP

    Immunofluorescent analysis of MCF-7 cells, using UROS antibody 14913-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human cervical cancer using 14913-1-AP

    IHC staining of human cervical cancer using 14913-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 14913-1-AP

    IHC staining of human cervical cancer using 14913-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14913-1-AP (UROS antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UROS Polyclonal antibody

UROS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14913-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Uroporphyrinogen-III synthase, Uroporphyrinogen-III cosynthase, uroporphyrinogen III synthase, UROIIIS, EC:4.2.1.75

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14913-1-AP targets UROS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6711

Product name: Recombinant human UROS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-265 aa of BC002573

Sequence: MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQPHGCC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uroporphyrinogen III synthase
别名 Uroporphyrinogen-III synthase, Uroporphyrinogen-III cosynthase, uroporphyrinogen III synthase, UROIIIS, EC:4.2.1.75
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC002573
基因名称 UROS
Gene ID (NCBI) 7390
RRIDAB_2213824
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10746
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for UROS antibody 14913-1-APDownload protocol
IHC protocol for UROS antibody 14913-1-APDownload protocol
IP protocol for UROS antibody 14913-1-APDownload protocol
WB protocol for UROS antibody 14913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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