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UROD Polyclonal antibody

UROD Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15547-1-AP

Print datasheet

Synonyms

PCT, UPD, URO D, UROD, uroporphyrinogen decarboxylase

验证数据展示

  • WB analysis using 15547-1-AP

    WB analysis using 15547-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15547-1-AP (UROD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 15547-1-AP

    IF Staining of HEK-293 using 15547-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using UROD antibody (15547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human kidney using 15547-1-AP

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 15547-1-AP

    IHC staining of human kidney using 15547-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15547-1-AP (UROD antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of K-562 using 15547-1-AP

    IP experiment of K-562 using 15547-1-AP

    IP result of anti-UROD (IP:15547-1-AP, 4ug; Detection:15547-1-AP 1:500) with K-562 cells lysate 4400 ug.

经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, HEK-293 cells, rat kidney tissue, mouse liver tissue, rat liver tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15547-1-AP targets UROD in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen UROD fusion protein Ag7905 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uroporphyrinogen decarboxylase
Synonyms PCT, UPD, URO D, UROD, uroporphyrinogen decarboxylase
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC001778
Gene Symbol UROD
Gene ID (NCBI) 7389
RRIDAB_2213813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06132
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UROD antibody 15547-1-APDownload protocol
IHC protocol for UROD antibody 15547-1-APDownload protocol
IF protocol for UROD antibody 15547-1-APDownload protocol
IP protocol for UROD antibody 15547-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
View Article