验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
25032-1-AP targets UROC1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag18143 Product name: Recombinant human UROC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 327-676 aa of BC115405 Sequence: HELDTTGECLVDLGSDQTSCHNPFNGGYYPVQLSFTEAQSLMASNPAVFKDLVQESLRRQVSAINRLAEEKFFFWDYGNAFLLEAQRAGADVEKKGAGRTEFRYPSYVQHIMGDIFSQGFGPFRWVCTSGDPQDLAVTDELATSVLEEAIADGVKVSVKLQYMDNIRWIREAARHRLVVGSQARILYSDQKGRVAIAVAINQAIACRRIKAPVVLSRDHHDVSGTDSPFRETSNIYDGSAFCADMAVQNFVGDACRGATWVALHNGGGVGWGEVINGGFGLVLDGTPEAEGRARLMLSWDVSNGVARRCWSGNQKAYEIICQTMQENSTLVVTLPHKVEDERVLQQALQL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | urocanase domain containing 1 |
| 别名 | HMFN0320, UROC1, Urocanase, urocanase domain containing 1, Urocanate hydratase |
| 计算分子量 | 676 aa, 75 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC115405 |
| 基因名称 | UROC1 |
| Gene ID (NCBI) | 131669 |
| RRID | AB_2879859 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96N76 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UROC1, also named as Urocanase and Imidazolonepropionate hydrolase, is involved in Urocanase deficiency (UROCD). UROC1 has some isoforms with MW 65-75 kDa and 80 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for UROC1 antibody 25032-1-AP | Download protocol |
| WB protocol for UROC1 antibody 25032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
