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验证数据展示

  • WB analysis using 15285-1-AP (same clone as 15285-1-PBS)

    WB analysis using 15285-1-AP (same clone as 15285-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 15285-1-AP (same clone as 15285-1-PBS)

    IHC staining of mouse liver using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 15285-1-AP (same clone as 15285-1-PBS)

    IHC staining of mouse liver using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15285-1-AP (URM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 15285-1-AP (same clone as 15285-1-PBS)

    WB analysis of human placenta using 15285-1-AP (same clone as 15285-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15285-1-AP (URM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocirrhosis using 15285-1-AP (same clone as 15285-1-PBS)

    IHC staining of human hepatocirrhosis using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • IHC staining of human hepatocirrhosis using 15285-1-AP (same clone as 15285-1-PBS)

    IHC staining of human hepatocirrhosis using 15285-1-AP (same clone as 15285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15285-1-AP (URM1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 15285-1-AP (same clone as 15285-1-PBS)

    IP experiment of HepG2 using 15285-1-AP (same clone as 15285-1-PBS)

    IP result of anti-URM1 (IP:15285-1-AP, 4ug; Detection:15285-1-AP 1:500) with HepG2 cells lysate 3360 ug. This data was developed using the same antibody clone with 15285-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

URM1 Polyclonal antibody, PBS Only

URM1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15285-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

C9orf74, Ubiquitin-related modifier 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15285-1-PBS targets URM1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7448

Product name: Recombinant human URM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-101 aa of BC003581

Sequence: MAAPLSVEVEFGGGAELLFDGIKKHRVTLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSVRPGILVLINDADWELLGELDYQLQDQDSVLFISTLHGG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin related modifier 1 homolog (S. cerevisiae)
别名 C9orf74, Ubiquitin-related modifier 1
计算分子量 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC003581
基因名称 URM1
Gene ID (NCBI) 81605
RRIDAB_2213808
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTM9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Urm1 (ubiquitin related modifier 1) is a molecular fossil in the class of ubiquitin-like proteins (UBLs). It encompasses characteristics of classical UBLs, such as ubiquitin or SUMO (small ubiquitin-related modifier), but also of bacterial sulfur-carrier proteins (SCP) (PMID: 33499055). By the assistance of an E1-like activation enzyme Uba4, Urm1 can function as a modifier to target proteins, called urmylation (PMID: 33548388). The ubiquitin-like protein Urm1 can be covalently conjugated to other proteins, such as the yeast thioredoxin peroxidase protein Ahp1p, through a mechanism involving the ubiquitin E1-like enzyme Uba4 (PMID: 19047990).

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