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URG4 Monoclonal antibody

URG4 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D5F12

Cat No : 67144-1-Ig

Print datasheet

Synonyms

KIAA1507, up regulated gene 4, URG4, URGCP

验证数据展示

  • IHC staining of human breast cancer using 67144-1-Ig

    IHC staining of human breast cancer using 67144-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67144-1-Ig

    IHC staining of human breast cancer using 67144-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 67144-1-Ig

    WB analysis using 67144-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67144-1-Ig (URG4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67144-1-Ig

    IF Staining of HeLa using 67144-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using URG4 antibody (67144-1-Ig, Clone: 2D5F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inSGC-7901 cells, MKN-45 cells, HepG2 cells, HuH-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human nasopharyngeal carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67144-1-Ig targets URG4 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen URG4 fusion protein Ag28635 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name up-regulated gene 4
Synonyms KIAA1507, up regulated gene 4, URG4, URGCP
Calculated Molecular Weight 922 aa, 104 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC018426
Gene Symbol URG4
Gene ID (NCBI) 55665
RRIDAB_2882443
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TCY9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.

实验方案

Product Specific Protocols
WB protocol for URG4 antibody 67144-1-IgDownload protocol
IHC protocol for URG4 antibody 67144-1-IgDownload protocol
IF protocol for URG4 antibody 67144-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols