验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
20023-1-AP targets URB1-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | URB1 ribosome biogenesis 1 homolog (S. cerevisiae) |
| 别名 | C21orf108, KIAA0539, URB1 |
| 计算分子量 | 254 kDa |
| 观测分子量 | 240-260 kDa |
| GenBank蛋白编号 | NM_014825 |
| 基因名称 | URB1 |
| Gene ID (NCBI) | 9875 |
| RRID | AB_10667000 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60287 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
URB1, also named as C21orf108, KIAA0539 and NPA1, is a 2,271 amino acid protein that is homologous to the S. cerevisiae protein URB1 which is a nucleolar protein involved in the normal accumulation of 25S and 5.8S rRNAs and may also play a role in the biosynthesis of the 60S ribosomal unit. Also localized to the nucleolus, human URB1 is thought to have the same functions as S. cerevisiae URB1. The antibody is specific to URB1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for URB1-Specific antibody 20023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance
| ||
FEBS Open Bio Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo |
