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验证数据展示

  • WB analysis using 14937-1-AP (same clone as 14937-1-PBS)

    WB analysis using 14937-1-AP (same clone as 14937-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14937-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 14937-1-AP (same clone as 14937-1-PBS)

    IHC staining of mouse kidney using 14937-1-AP (same clone as 14937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14937-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 14937-1-AP (same clone as 14937-1-PBS)

    IHC staining of mouse kidney using 14937-1-AP (same clone as 14937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14937-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 14937-1-AP (same clone as 14937-1-PBS)

    IHC staining of human kidney using 14937-1-AP (same clone as 14937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14937-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 14937-1-AP (same clone as 14937-1-PBS)

    IHC staining of human kidney using 14937-1-AP (same clone as 14937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14937-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 14937-1-AP (same clone as 14937-1-PBS)

    IF Staining of mouse kidney using 14937-1-AP (same clone as 14937-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using URAT1 antibody (14937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14937-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 14937-1-AP (same clone as 14937-1-PBS)

    IF Staining of mouse kidney using 14937-1-AP (same clone as 14937-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using URAT1 antibody (14937-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14937-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

URAT1 Polyclonal antibody, PBS Only

URAT1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 14937-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

SLC22A12, OAT4L, OATL4, Organic anion transporter 4-like protein, Renal specific transporter

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14937-1-PBS targets URAT1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag6770

Product name: Recombinant human URAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4-351 aa of BC053348

Sequence: SELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 22 (organic anion/urate transporter), member 12
别名 SLC22A12, OAT4L, OATL4, Organic anion transporter 4-like protein, Renal specific transporter
计算分子量 60 kDa
观测分子量 65-70 kDa, 40 kDa
GenBank蛋白编号BC053348
基因名称 URAT1
Gene ID (NCBI) 116085
RRIDAB_2191270
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96S37
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the native and glycosylated URAT1 between 65 and 70 kDa as well as the truncated 40kDa protein.

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