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验证数据展示

  • WB analysis using 87199-1-RR (same clone as 87199-1-PBS)

    WB analysis using 87199-1-RR (same clone as 87199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87199-1-RR (UQCRQ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 87199-1-RR (same clone as 87199-1-PBS)

    IHC staining of mouse heart using 87199-1-RR (same clone as 87199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 87199-1-RR (UQCRQ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UQCRQ antibody included
  • IHC staining of rat liver using 87199-1-RR (same clone as 87199-1-PBS)

    IHC staining of rat liver using 87199-1-RR (same clone as 87199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 87199-1-RR (UQCRQ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UQCRQ antibody included
  • IF Staining of HepG2 using 87199-1-RR (same clone as 87199-1-PBS)

    IF Staining of HepG2 using 87199-1-RR (same clone as 87199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRQ antibody (87199-1-RR, Clone: 252364C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87199-1-PBS in a different storage buffer formulation.

  • Affinity of 87199-1-RR

    Affinity of 87199-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87199-1-RR against Human UQCRQ were performed. The affinity constant is 2.79 nM.

UQCRQ Recombinant monoclonal antibody, PBS Only

UQCRQ Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87199-1-PBS

产品说明书

CloneNo. 252364C10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Complex III subunit 8, Complex III subunit VIII, Cytochrome b-c1 complex subunit 8, QCR8, QP C

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy UQCRQ Ready-To-Use IHC Kit. UQCRQ primary antibody included.

产品信息

87199-1-PBS targets UQCRQ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6907

Product name: Recombinant human UQCRQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-82 aa of BC001390

Sequence: MGREFGNLTRMRHVISYSLSPFEQRAYPHVFTKGIPNVLRRIRESFFRVVPQFVVFYLIYTWGTEEFERSKRKNPAAYENDK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa
别名 Complex III subunit 8, Complex III subunit VIII, Cytochrome b-c1 complex subunit 8, QCR8, QP C
计算分子量 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC001390
基因名称 UQCRQ
Gene ID (NCBI) 27089
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14949
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.

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