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验证数据展示

  • IHC staining of human breast cancer using 12364-1-AP

    IHC staining of human breast cancer using 12364-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12364-1-AP (UQCRH antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UQCRH antibody included
  • IHC staining of human breast cancer using 12364-1-AP

    IHC staining of human breast cancer using 12364-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12364-1-AP (UQCRH antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UQCRH antibody included

UQCRH Polyclonal antibody

UQCRH Polyclonal Antibody for IHC, ELISA
Cat No. 12364-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA and More (1)

别名

Complex III subunit 6, Complex III subunit VIII, Mitochondrial hinge protein, QCR6, UQCRH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UQCRH Ready-To-Use IHC Kit. UQCRH primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12364-1-AP targets UQCRH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3037

Product name: Recombinant human UQCRH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC015177

Sequence: MGLEDEQKMLTESGDPEEEEEEEEELVDPLTTVREQCEQLEKCVKARERLELCDERVSSRSHTEEDCTEELFDFLHARDHCVAHKLFNNLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquinol-cytochrome c reductase hinge protein
别名 Complex III subunit 6, Complex III subunit VIII, Mitochondrial hinge protein, QCR6, UQCRH
计算分子量 91 aa, 11 kDa
GenBank蛋白编号BC015177
基因名称 UQCRH
Gene ID (NCBI) 7388
RRIDAB_2877850
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07919
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UQCRH(ubiquinol-cytochrome c reductase hinge protein) belongs to the UQCRH/QCR6 family. It is a part of the mitochondrial respiratory chain that may mediate formation of the complex between cytochromes c and c1. The deduced 91-amino acid protein has a 13-amino acid N-terminal mitochondrial targeting presequence.

实验方案

Product Specific Protocols
IHC protocol for UQCRH antibody 12364-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Adv Sci (Weinh)

Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption

Authors - Fan Ouyang
View Article