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验证数据展示

  • WB analysis using 88500-1-RR

    WB analysis using 88500-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88500-1-RR (UQCRFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 88500-1-RR

    IP experiment of HeLa using 88500-1-RR

    IP result of anti-UQCRFS1 (IP:88500-1-RR, 4ug; Detection:88500-1-RR 1:3000) with HeLa cells lysate 1080 ug.

  • IF Staining of MCF-7 using 88500-1-RR

    IF Staining of MCF-7 using 88500-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using UQCRFS1 antibody (88500-1-RR, Clone: 261156A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

UQCRFS1 Recombinant monoclonal antibody

UQCRFS1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 88500-1-RR

产品说明书

CloneNo. 261156A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Complex III subunit 5, Complex III subunit IX, Cytochrome b-c1 complex subunit 5, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit Rieske, mitochondrial

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, A2780 cells, mouse heart tissue, rat heart tissue, human heart tissue, human placenta
Positive IP detected inHeLa cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy UQCRFS1 Ready-To-Use IHC Kit. UQCRFS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88500-1-RR targets UQCRFS1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13315

Product name: Recombinant human UQCRFS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-274 aa of BC010035

Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
别名 Complex III subunit 5, Complex III subunit IX, Cytochrome b-c1 complex subunit 5, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit Rieske, mitochondrial
计算分子量 30 kDa
观测分子量 25 kDa
GenBank蛋白编号BC010035
基因名称 UQCRFS1
Gene ID (NCBI) 7386
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP47985
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.

实验方案

Product Specific Protocols
IF protocol for UQCRFS1 antibody 88500-1-RRDownload protocol
IP protocol for UQCRFS1 antibody 88500-1-RRDownload protocol
WB protocol for UQCRFS1 antibody 88500-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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