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Host
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Species Reactivity
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 83667-2-RR (same clone as 83667-2-PBS)

    WB analysis using 83667-2-RR (same clone as 83667-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83667-2-RR (UQCRC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83667-2-RR (same clone as 83667-2-PBS)

    IHC staining of human stomach cancer using 83667-2-RR (same clone as 83667-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83667-2-RR (UQCRC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UQCRC2 antibody included
  • FC experiment of HepG2 using 83667-2-RR (same clone as 83667-2-PBS)

    FC experiment of HepG2 using 83667-2-RR (same clone as 83667-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug UQCRC2 Recombinant antibody (83667-2-RR, Clone:240454D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83667-2-RR

    Affinity and Kinetic Characterization of 83667-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83667-2-RR against Human UQCRC2 were performed. The affinity constant is 21.8 nM.

    {{ptg:ProductFeaturesInfo}}

UQCRC2 Recombinant monoclonal antibody, PBS Only

UQCRC2 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83667-2-PBS

产品说明书

CloneNo. 240454D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy UQCRC2 Ready-To-Use IHC Kit. UQCRC2 primary antibody included.

产品信息

83667-2-PBS targets UQCRC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6432

Product name: Recombinant human UQCRC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 103-453 aa of BC000484

Sequence: IEAVGGKLSVTATRENMAYTVECLRGDVDILMEFLLNVTTAPEFRRWEVADLQPQLKIDKAVAFQNPQTHVIENLHAAAYRNALANPLYCPDYRIGKVTSEELHYFVQNHFTSARMALIGLGVSHPVLKQVAEQFLNMRGGLGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDVIKAAYNQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSECFLEEVGSQALVAGSYMPPSTVLQQIDSVANADIINAAKKFVSGQKSMAASGNLGHTPFVDEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquinol-cytochrome c reductase core protein II
别名 Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000484
基因名称 UQCRC2
Gene ID (NCBI) 7385
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP22695
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.