验证数据展示
经过测试的应用
| Positive IP detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12964-1-AP targets UPP2 in IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3619 Product name: Recombinant human UPP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC033529 Sequence: MASVIPASNRSMRSDRNTYVGKRFVHVKNPYLDLMDEDILYHLDLGTKTHNLPAMFGDVKFVCVGGSPNRMKAFALFMHKELGFEEAEEDIKDICAGTDRYCMYKTGPVLAISHGMGIPSISIMLHELIKLLHHARCCDVTIIRIGTSGGIGIAPGTVVITDIAVDSFFKPRFEQVILDNIVTRSTELDKELSEELFNCSKEIPNFPTLVGHTMCTYDFYEGQGRLDGALCSFSREKKLDYLKRAFKAGVRNIEMESTVFAAMCGLCGLKAAVVCVTLLDRLDCDQINLPHDVLVEYQQRPQLLISNFIRRRLGLCD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | uridine phosphorylase 2 |
| 别名 | EC:2.4.2.3, UDRPASE2, UP2, UPase 2, UPASE2 |
| 计算分子量 | 317 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC033529 |
| 基因名称 | UPP2 |
| Gene ID (NCBI) | 151531 |
| RRID | AB_3741836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95045 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Uridine phosphorylase (UPP) catalyzes the reversible phosphorolysis of uridine and chemically-related compounds to uracil and ribose-1-phosphate, implicating it in both pyrimidine salvage and regulation of uridine homeostasis .The enzyme is ubiquitously distributed amongst animals and bacteria, with most vertebrates possessing two versions of the phosphorylase, UPP1 and UPP2. While the two human homologues retain ~62% sequence identity, human UPP1 (hUPP1) was the first identified, cloned, and characterized, and consequently has been the focus of the majority of research . Not as much is known about the specific role of human UPP2 (hUPP2). Early studies showed that its tissue distribution was more limited than that of hUPP1, restricted predominantly to the kidneys, with some expression in the liver and spleen . (PMID: 21855639, PMID: 37473754)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for UPP2 antibody 12964-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
