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验证数据展示

  • WB analysis using 55229-1-AP

    WB analysis using 55229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55229-1-AP (UPK3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse bladder using 55229-1-AP

    IHC staining of mouse bladder using 55229-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 55229-1-AP (Uroplakin III antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UPK3A Polyclonal antibody

UPK3A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55229-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Uroplakin III, uroplakin 3A, UPK3, UPIIIA, UPIII

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue
Positive IHC detected inmouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55229-1-AP targets UPK3A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uroplakin 3A
别名 Uroplakin III, uroplakin 3A, UPK3, UPIIIA, UPIII
计算分子量 31 kDa
观测分子量31-33 kDa
GenBank蛋白编号NM_006953
基因名称 UPK3A
Gene ID (NCBI) 7380
RRIDAB_3670244
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75631
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for UPK3A antibody 55229-1-APDownload protocol
IHC protocol for UPK3A antibody 55229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols