UPK1A Polyclonal antibody
UPK1A Polyclonal Antibody for IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IP detected in | mouse bladder tissue |
| Positive IHC detected in | human bladder tissue, rat bladder tissue, mouse bladder tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
The immunogen of 25275-1-AP is UPK1A Fusion Protein expressed in E. coli.
| Tested Applications | IHC, IP, ELISA |
| Cited Applications | IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | UPK1A fusion protein Ag17356 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | uroplakin 1A |
| Synonyms | Tetraspanin 21, Tspan 21, TSPAN21, UP1A, UPIA, UPK1A, UPKA, uroplakin 1A, Uroplakin Ia |
| Calculated Molecular Weight | 258 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC107098 |
| Gene Symbol | UPK1A |
| Gene ID (NCBI) | 11045 |
| RRID | AB_2880000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00322 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1a (UPK1A) belongs to the tetraspanin (TM4SF) family. UPK1A plays an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for UPK1A antibody 25275-1-AP | Download protocol |
| IP protocol for UPK1A antibody 25275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Stem Cells Int Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway. | ||
Int J Urol Differential effects of adipose tissue stromal cells on the apoptosis, growth and invasion of bladder urothelial carcinoma between the superficial and invasive types. |
