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验证数据展示

  • WB analysis using 28384-1-AP

    WB analysis using 28384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28384-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 28384-1-AP

    WB analysis of HEK-293 using 28384-1-AP

    WB result of UPF1 antibody (28384-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UPF1 transfected HEK-293 cells.

  • IHC staining of human breast cancer using 28384-1-AP

    IHC staining of human breast cancer using 28384-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UPF1 antibody included
  • IHC staining of human breast cancer using 28384-1-AP

    IHC staining of human breast cancer using 28384-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UPF1 antibody included
  • IP experiment of HeLa using 28384-1-AP

    IP experiment of HeLa using 28384-1-AP

    IP result of anti-UPF1 (IP:28384-1-AP, 4ug; Detection:28384-1-AP 1:800) with HeLa cells lysate 1440 ug.

  • IF Staining of HepG2 using 28384-1-AP

    IF Staining of HepG2 using 28384-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UPF1 antibody (28384-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

UPF1 Polyclonal antibody

UPF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 28384-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

RENT1, ATP-dependent helicase RENT1, EC:3.6.4.12, KIAA0221, Regulator of nonsense transcripts 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Raji cells, SH-SY5Y cells, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy UPF1 Ready-To-Use IHC Kit. UPF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28384-1-AP targets UPF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29057

Product name: Recombinant human UPF1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 310-607 aa of BC039817

Sequence: VKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UPF1 regulator of nonsense transcripts homolog (yeast)
别名 RENT1, ATP-dependent helicase RENT1, EC:3.6.4.12, KIAA0221, Regulator of nonsense transcripts 1
计算分子量123 kDa
观测分子量 123-130 kDa
GenBank蛋白编号BC039817
基因名称 UPF1
Gene ID (NCBI) 5976
RRIDAB_2881130
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

实验方案

Product Specific Protocols
IF protocol for UPF1 antibody 28384-1-APDownload protocol
IHC protocol for UPF1 antibody 28384-1-APDownload protocol
IP protocol for UPF1 antibody 28384-1-APDownload protocol
WB protocol for UPF1 antibody 28384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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