- Featured Product
- KD/KO Validated
UPF1 Polyclonal antibody
UPF1 Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IF/ICC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Raji cells, SH-SY5Y cells |
Positive IP detected in | Raji cells |
Positive IF detected in | HepG2 cells, MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
23379-1-AP targets UPF1 in WB, IP, IF, RIP, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, IP, WB, ELISA |
Cited Applications | WB, IF, RIP |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | UPF1 fusion protein Ag16195 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | UPF1 regulator of nonsense transcripts homolog (yeast) |
Synonyms | ATP dependent helicase RENT1, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1 |
Calculated Molecular Weight | 123 kDa |
Observed Molecular Weight | 123-130 kDa |
GenBank Accession Number | BC039817 |
Gene Symbol | UPF1 |
Gene ID (NCBI) | 5976 |
RRID | AB_11232421 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92900 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for UPF1 antibody 23379-1-AP | Download protocol |
IF protocol for UPF1 antibody 23379-1-AP | Download protocol |
IP protocol for UPF1 antibody 23379-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
iScience Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1 | ||
Breast Cancer Res Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1. | ||
Bioengineered The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells. | ||
J Exp Clin Cancer Res PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α. | ||
Cell Death Dis PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay. | ||
Cell Rep UPF1 reduces C9orf72 HRE-induced neurotoxicity in the absence of nonsense-mediated decay dysfunction.
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