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验证数据展示

  • WB analysis of mouse liver using 24603-1-AP

    WB analysis of mouse liver using 24603-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24603-1-AP (UPB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 24603-1-AP

    IHC staining of human heart using 24603-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24603-1-AP (UPB1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 24603-1-AP

    IHC staining of human heart using 24603-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24603-1-AP (UPB1 Antibody) at dilution of 1:50 (under 40x lens).

UPB1 Polyclonal antibody

UPB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24603-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Beta alanine synthase, Beta ureidopropionase, BUP 1, BUP1, UPB1, ureidopropionase, beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24603-1-AP targets UPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 UPB1 fusion protein Ag18960 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ureidopropionase, beta
别名 Beta alanine synthase, Beta ureidopropionase, BUP 1, BUP1, UPB1, ureidopropionase, beta
计算分子量 384 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC131703
基因名称 UPB1
Gene ID (NCBI) 51733
RRIDAB_2879634
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBR1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BUP1(Beta-ureidopropionase) is also named as BUP1, Beta-alanine synthase, N-carbamoyl-beta-alanine amidohydrolase and belongs to the CN hydrolase family. It catalyzes the last step in the pyrimidine degradation pathway that it converts N-carbamyl-beta-aminoisobutyric acid and N-carbamyl-beta-alanine to, respectively, beta-aminoisobutyric acid and beta-alanine, ammonia and carbon dioxide. It is a protein with a calculated molecular mass of 43 kDa.

实验方案

Product Specific Protocols
WB protocol for UPB1 antibody 24603-1-APDownload protocol
IHC protocol for UPB1 antibody 24603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols