验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
15000-1-AP targets UNKL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6990 Product name: Recombinant human UNKL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC000150 Sequence: MTCCSQVPPRRRPSLALSPRLDCNGLNGVPGSIWDFVSGSFSPSPSPILSAGPPSSSSASPNGAELARVRRQLDEAKRKIRQWEESWQQVKQVCDAWQREAQEAKERARVADSDRQLALQKKEEVEAQVKQLQEELEGLGVASTLPGLRGCGDIGTIPLPKLHSLQSQLRLDLEAVDGVIFQLRAKQCVACRERAHGAVLRPCQHHILCEPCAATAPECPYCKGQPLQW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | unkempt homolog (Drosophila)-like |
| 别名 | C16orf28, EC:2.3.2.-, KIAA0734, Putative E3 ubiquitin-protein ligase UNKL, RING finger protein unkempt-like |
| 计算分子量 | 25 kDa |
| 观测分子量 | 32 kDa, 45 kDa |
| GenBank蛋白编号 | BC000150 |
| 基因名称 | UNKL |
| Gene ID (NCBI) | 64718 |
| RRID | AB_2213189 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H9P5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Putative E3 ubiquitin-protein ligase UNKL was expressed at significantly lower levels in endometrial tumor tissues as compared to the endometrium. Importantly, in human endometrial cancer, primary tumor expression of UNKL was correlated with overall survival in black patients with high mutational burden.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UNKL antibody 15000-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Biother Radiopharm Tectonic 1 Is a Key Regulator of Cell Proliferation in Pancreatic Cancer. | ||
Toxicol Res (Camb) Celastrol inhibits gastric cancer cell proliferation, migration, and invasion via the FOXA1/CLDN4 axis |
