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验证数据展示

  • WB analysis using 12394-1-AP

    WB analysis using 12394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12394-1-AP (UNG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HuH-7 using 12394-1-AP

    WB analysis of HuH-7 using 12394-1-AP

    WB result of UNG antibody (12394-1-AP; 1:3000; room temperature for 1.5 hours) with wild-type and UNG knockout HuH-7 cells.

  • IHC staining of mouse heart using 12394-1-AP

    IHC staining of mouse heart using 12394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12394-1-AP (UNG antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNG antibody included
  • IHC staining of mouse heart using 12394-1-AP

    IHC staining of mouse heart using 12394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12394-1-AP (UNG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNG antibody included
  • IHC staining of mouse heart using 12394-1-AP

    IHC staining of mouse heart using 12394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12394-1-AP (UNG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNG antibody included
  • IF Staining of HeLa using 12394-1-AP

    IF Staining of HeLa using 12394-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12394-1-AP (UNG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

UNG Polyclonal antibody

UNG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12394-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Uracil-DNA glycosylase, UNG1, EC:3.2.2.27, DGU

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, HuH-7 cells, HeLa cells
Positive IHC detected inmouse heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UNG Ready-To-Use IHC Kit. UNG primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12394-1-AP targets UNG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag3072

Product name: Recombinant human UNG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-304 aa of BC015205

Sequence: MGVFCLGPWGLGRKLRTPGKGPLQLLSRLCGDHLQAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDWKEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uracil-DNA glycosylase
别名 Uracil-DNA glycosylase, UNG1, EC:3.2.2.27, DGU
计算分子量 304 aa, 34 kDa
观测分子量 28 kDa, 34-40 kDa
GenBank蛋白编号BC015205
基因名称 UNG
Gene ID (NCBI) 7374
RRIDAB_10734597
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13051
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930). Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).

实验方案

Product Specific Protocols
IF protocol for UNG antibody 12394-1-APDownload protocol
IHC protocol for UNG antibody 12394-1-APDownload protocol
WB protocol for UNG antibody 12394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates

Authors - Yuan Fu
View Article
humanWB

Mol Cell

Uracil processing by SMUG1 in the absence of UNG triggers homologous recombination and selectively kills BRCA1/2-deficient tumors

Authors - Daniele Musiani
View Article
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