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验证数据展示

  • WB analysis using 12394-1-AP

    WB analysis using 12394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12394-1-AP (UNG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HuH-7 using 12394-1-AP

    WB analysis of HuH-7 using 12394-1-AP

    WB result of UNG antibody (12394-1-AP; 1:3000; room temperature for 1.5 hours) with wild-type and UNG knockout HuH-7 cells.

  • IHC staining of human testis using 12394-1-AP

    IHC staining of human testis using 12394-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12394-1-AP (UNG antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNG antibody included
  • IHC staining of human testis using 12394-1-AP

    IHC staining of human testis using 12394-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12394-1-AP (UNG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNG antibody included
  • IF Staining of HeLa using 12394-1-AP

    IF Staining of HeLa using 12394-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UNG antibody (12394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

UNG Polyclonal antibody

UNG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12394-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

DGU, EC:3.2.2.27, UNG1, Uracil-DNA glycosylase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, HuH-7 cells, HeLa cells
Positive IHC detected inhuman testis tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UNG Ready-To-Use IHC Kit. UNG primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12394-1-AP targets UNG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag3072

Product name: Recombinant human UNG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-304 aa of BC015205

Sequence: MGVFCLGPWGLGRKLRTPGKGPLQLLSRLCGDHLQAIPAKKAPAGQEEPGTPPSSPLSAEQLDRIQRNKAAALLRLAARNVPVGFGESWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDWKEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 uracil-DNA glycosylase
别名 DGU, EC:3.2.2.27, UNG1, Uracil-DNA glycosylase
计算分子量 304 aa, 34 kDa
观测分子量 28 kDa, 34-40 kDa
GenBank蛋白编号BC015205
基因名称 UNG
Gene ID (NCBI) 7374
RRIDAB_10734597
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13051
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930). Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).

实验方案

Product Specific Protocols
IF protocol for UNG antibody 12394-1-APDownload protocol
IHC protocol for UNG antibody 12394-1-APDownload protocol
WB protocol for UNG antibody 12394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Uracil processing by SMUG1 in the absence of UNG triggers homologous recombination and selectively kills BRCA1/2-deficient tumors

Authors - Daniele Musiani
View Article

Nucleic Acids Res

Human PMS1-dependent non-canonical mismatch repair engages with MBD4 to repair methylated CpG deamination.

Authors - Anaïs Le Ven
View Article
humanWB

Dev Cell

Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates

Authors - Yuan Fu
View Article
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