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UNC5C-Specific Polyclonal antibody

UNC5C-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20240-1-AP

Print datasheet

Synonyms

Netrin receptor UNC5C, Protein unc 5 homolog 3, Protein unc 5 homolog C, unc 5 homolog C (C. elegans), UNC5C, UNC5C-Specific, UNC5H3

验证数据展示

  • WB analysis using 20240-1-AP

    WB analysis using 20240-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20240-1-AP

    IHC staining of mouse brain using 20240-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20240-1-AP

    IHC staining of mouse brain using 20240-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse kidney tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20240-1-AP targets UNC5C-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name unc-5 homolog C (C. elegans)
Synonyms Netrin receptor UNC5C, Protein unc 5 homolog 3, Protein unc 5 homolog C, unc 5 homolog C (C. elegans), UNC5C, UNC5C-Specific, UNC5H3
Calculated Molecular Weight 103 kDa
Observed Molecular Weight 50 kDa, 103 kDa
GenBank Accession NumberNM_003728
Gene Symbol UNC5C
Gene ID (NCBI) 8633
RRIDAB_10695632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95185
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC5C, also named as UNC5H3, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5C.

实验方案

Product Specific Protocols
WB protocol for UNC5C-Specific antibody 20240-1-APDownload protocol
IHC protocol for UNC5C-Specific antibody 20240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Alcohol Clin Exp Res

Fetal alcohol exposure impairs learning and memory functions and elevates levels of various biochemical markers of Alzheimer's disease in the brain of twelve-month-old rats

Authors - Shaista Chaudhary
View Article