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UNC5A-Specific Polyclonal antibody

UNC5A-Specific Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20239-1-AP

Print datasheet

Synonyms

KIAA1976, Netrin receptor UNC5A, Protein unc 5 homolog 1, Protein unc 5 homolog A, unc 5 homolog A (C. elegans), UNC5A, UNC5H1

验证数据展示

  • WB analysis of mouse brain using 20239-1-AP

    WB analysis of mouse brain using 20239-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 20239-1-AP

    FC experiment of MCF-7 using 20239-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug UNC5A-Specific antibody (20239-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive FC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20239-1-AP targets UNC5A-Specific in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name unc-5 homolog A (C. elegans)
Synonyms KIAA1976, Netrin receptor UNC5A, Protein unc 5 homolog 1, Protein unc 5 homolog A, unc 5 homolog A (C. elegans), UNC5A, UNC5H1
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberNM_133369
Gene Symbol UNC5A
Gene ID (NCBI) 90249
RRIDAB_10695050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZN44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5A. It has no cross reaction to UNC5B, UNC5C and UNC5D.

实验方案

Product Specific Protocols
WB protocol for UNC5A-Specific antibody 20239-1-APDownload protocol
FC protocol for UNC5A-Specific antibody 20239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols