验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervix tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy UNC45A Ready-To-Use IHC Kit. UNC45A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15479-1-AP targets UNC45A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, fish |
| 免疫原 |
CatNo: Ag7790 Product name: Recombinant human UNC45A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 722-944 aa of BC006214 Sequence: EMTFPGERIYEVVRPLVSLLHLNCSGLQNFEALMALTNLAGISERLRQKILKEKAVPMIEGYMFEEHEMIRRAATECMCNLAMSKEVQDLFEAQGNDRLKLLVLYSGEDDELLQRAAAGGLAMLTSMRPTLCSRIPQVTTHWLEILQALLLSSNQELQHRGAVVVLNMVEASREIASTLMESEMMEILSVLAKGDHSPVTRAAAACLDKAVEYGLIQPNQDGE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | unc-45 homolog A (C. elegans) |
| 别名 | GC UNC45, GCUNC 45, GCUNC45, IRO039700, Protein unc 45 homolog A, SMAP 1, SMAP1, unc 45 homolog A (C. elegans), Unc 45A, UNC45A |
| 计算分子量 | 103 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC006214 |
| 基因名称 | UNC45A |
| Gene ID (NCBI) | 55898 |
| RRID | AB_2212736 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H3U1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for UNC45A antibody 15479-1-AP | Download protocol |
| IHC protocol for UNC45A antibody 15479-1-AP | Download protocol |
| IP protocol for UNC45A antibody 15479-1-AP | Download protocol |
| WB protocol for UNC45A antibody 15479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.
| ||
Food Res Int Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella). | ||
Cancer Biol Ther UNC-45A is preferentially expressed in epithelial cells and binds to and co-localizes with interphase MTs. | ||
J Biol Chem Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors | ||
Gastroenterology Myosin Vb Traffics P-glycoprotein to the Apical Membrane of Intestinal Epithelial Cells |
