验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, K-562 cells, Jurkat cells, HL-60 cells |
| Positive IHC detected in | rat spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy UNC13D Ready-To-Use IHC Kit. UNC13D primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
67193-1-Ig targets UNC13D in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat, pig |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag10497 Product name: Recombinant human UNC13D protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-327 aa of BC067084 Sequence: MATLLSHPQQRPPFLRQAIKIRRRRVRDLQDPPPQMAPEIQPPSHHFSPEQRALLYEDALYTVLHRLGHPEPNHVTEASELLRYLQEAFHVEPEEHQQTLQRVRELEKPIFCLKATVKQAKGILGKDVSGFSDPYCLLGIEQGVGVPGGSPGSRHRQKAVVRHTIPEEETHRTQVITQTLNPVWDETFILEFEDITNASFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPRTETYPDRGQCHLQFQLIHKRRATSASRSQPSYTVHLHLLQQLVSHEVTQHEAGSTSWDGSL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | unc-13 homolog D (C. elegans) |
| 别名 | FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D |
| 计算分子量 | 1090 aa, 103 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC067084 |
| 基因名称 | UNC13D |
| Gene ID (NCBI) | 201294 |
| RRID | AB_2882488 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q70J99 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for UNC13D antibody 67193-1-Ig | Download protocol |
| WB protocol for UNC13D antibody 67193-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
