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验证数据展示

  • IF Staining of SH-SY5Y using 83000-6-RR

    IF Staining of SH-SY5Y using 83000-6-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using UNC13A antibody (83000-6-RR, Clone: 230469G2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of rat brain using 83000-6-RR

    IF Staining of rat brain using 83000-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using UNC13A antibody (83000-6-RR, Clone: 230469G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 83000-6-RR

    IF Staining of rat brain using 83000-6-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using UNC13A antibody (83000-6-RR, Clone: 230469G2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UNC13A Recombinant antibody

UNC13A Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, ELISA
Cat No. 83000-6-RR

产品说明书

CloneNo. 230469G2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IF/ICC, IF-P, ELISA

别名

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83000-6-RR targets UNC13A in IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 unc-13 homolog A (C. elegans)
别名 Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032
计算分子量 193 kDa
GenBank蛋白编号NM_001080421
基因名称 UNC13A
Gene ID (NCBI) 23025
RRIDAB_3670747
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UPW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

实验方案

Product Specific Protocols
IF protocol for UNC13A antibody 83000-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols