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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83000-5-RR (same clone as 83000-5-PBS)

    WB analysis using 83000-5-RR (same clone as 83000-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83000-5-RR (UNC13A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    IP experiment of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    IP result of anti-UNC13A (IP:83000-5-RR, 4ug; Detection:83000-5-RR 1:1000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    IHC staining of mouse brain using 83000-5-RR (same clone as 83000-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83000-5-PBS in a different storage buffer formulation.

UNC13A Recombinant antibody, PBS Only

UNC13A Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 83000-5-PBS

产品说明书

CloneNo. 240017A6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IP, Indirect ELISA

别名

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83000-5-PBS targets UNC13A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 unc-13 homolog A (C. elegans)
别名 Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032
计算分子量 193 kDa
观测分子量193-210 kDa
GenBank蛋白编号NM_001080421
基因名称 UNC13A
Gene ID (NCBI) 23025
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UPW8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.