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UNC13A/Munc13-1 Polyclonal antibody

UNC13A/Munc13-1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 55053-1-AP

Print datasheet

Synonyms

KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A, UNC13A/Munc13-1

验证数据展示

  • WB analysis using 55053-1-AP

    WB analysis using 55053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 55053-1-AP

    IP experiment of mouse brain using 55053-1-AP

    IP result of anti-UNC13A/Munc13-1 (IP:55053-1-AP, 4ug; Detection:55053-1-AP 1:500) with mouse brain tissue lysate 5200ug.

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55053-1-AP targets UNC13A/Munc13-1 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name unc-13 homolog A (C. elegans)
Synonyms KIAA1032, Munc13 1, Protein unc 13 homolog A, unc 13 homolog A (C. elegans), UNC13A, UNC13A/Munc13-1
Calculated Molecular Weight 193 kDa
Observed Molecular Weight 193-210 kDa
GenBank Accession NumberNM_001080421
Gene Symbol UNC13A
Gene ID (NCBI) 23025
RRIDAB_10804173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.

实验方案

Product Specific Protocols
WB protocol for UNC13A/Munc13-1 antibody 55053-1-APDownload protocol
IP protocol for UNC13A/Munc13-1 antibody 55053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.

Authors - X Rosa Ma
View Article
mouseWB

Elife

Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa.

Authors - Fushun Fan
View Article
CoIP

Cell Rep

Structural and Functional Analysis of the CAPS SNARE-Binding Domain Required for SNARE Complex Formation and Exocytosis.

Authors - Hao Zhou
View Article