验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, Y79 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26535-1-AP targets UNC119B in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | UNC119B fusion protein Ag24916 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | unc-119 homolog B (C. elegans) |
| 别名 | Protein unc-119 homolog B |
| 观测分子量 | 28-32 kDa |
| GenBank蛋白编号 | BC004815 |
| 基因名称 | UNC119B |
| Gene ID (NCBI) | 84747 |
| RRID | AB_3669546 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A6NIH7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. The myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962). Mouse Unc119b shares 90% sequence identity with human UNC119b protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UNC119B antibody 26535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |