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验证数据展示

  • WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

    WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

    IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ULK1 antibody included
  • IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

    IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ULK1 antibody included
  • IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

    IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ULK1 antibody included
  • IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

    IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ULK1 antibody included

ULK1 Monoclonal antibody, PBS Only

ULK1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68445-1-PBS

产品说明书

CloneNo. 1D7G1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

1D7G1, ATG1, Autophagy-related protein 1 homolog, EC:2.7.11.1, hATG1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

产品信息

68445-1-PBS targets ULK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22397

Product name: Recombinant human ULK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 17-466 aa of NM_003565

Sequence: SRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 unc-51-like kinase 1 (C. elegans)
别名 1D7G1, ATG1, Autophagy-related protein 1 homolog, EC:2.7.11.1, hATG1
观测分子量 130-150 kDa
GenBank蛋白编号NM_003565
基因名称 ULK1
Gene ID (NCBI) 8408
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO75385
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

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