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验证数据展示

  • WB analysis of mouse colon using 13642-1-AP

    WB analysis of mouse colon using 13642-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13642-1-AP

    IF Staining of HeLa using 13642-1-AP

    Immunofluorescent analysis of Hela cells, using UIMC1 antibody 13642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of COLO 320 using 13642-1-AP

    IP experiment of COLO 320 using 13642-1-AP

    IP result of anti-UIMC1 (IP:13642-1-AP, 4ug; Detection:13642-1-AP 1:500) with COLO 320 cells lysate 2400ug.

  • IHC staining of human testis using 13642-1-AP

    IHC staining of human testis using 13642-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 13642-1-AP

    IHC staining of human testis using 13642-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 40x lens).

UIMC1 Polyclonal antibody

UIMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13642-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

BRCA1-A complex subunit RAP80, RAP80, Receptor-associated protein 80, Retinoid X receptor-interacting protein 110, RXRIP110

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue, COLO 320 cells, HeLa cells, Raji cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13642-1-AP targets UIMC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4582

Product name: Recombinant human UIMC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 202-553 aa of BC032561

Sequence: DQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin interaction motif containing 1
别名 BRCA1-A complex subunit RAP80, RAP80, Receptor-associated protein 80, Retinoid X receptor-interacting protein 110, RXRIP110
计算分子量 719 aa, 80 kDa
观测分子量 97 kDa, 80 kDa, 71-75 kDa, 61 kDa
GenBank蛋白编号BC032561
基因名称 UIMC1
Gene ID (NCBI) 51720
RRIDAB_10641052
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RL1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for UIMC1 antibody 13642-1-APDownload protocol
IHC protocol for UIMC1 antibody 13642-1-APDownload protocol
IP protocol for UIMC1 antibody 13642-1-APDownload protocol
WB protocol for UIMC1 antibody 13642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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