验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24345-1-AP targets UHRF1BP1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag19677 Product name: Recombinant human UHRF1BP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1053-1400 aa of BC137120 Sequence: LGRDRMTSTMHKMLSLPPAKEPMAKTDEGVAAPVSGGAARLRFFSMKRTVSQQSFDGVSLDSSGPEDRISVDSDGSDSFVMLLESESGPESVPPGSLSNVSDNAGVQGSPLVNNYGQGSPAANSSVSPSGEDLIFHPVSVLVLKVNEVSFGIEVRGEDLTVALQAEELTLQQLGTVGLWQFLHGQCPGTCFQESSTLKTGHIRPAVGLRFEVGPGAAVHSPLASQNGFLHLLLHGCDLELLTSVLSGLGPFLEDEEIPVVVPMQIELLNSSITLKDDIPPIYPTSPGPIPITLAMEHVVLKRSDDGVFHIGAAAQDKPSAEVLKSEKRQPPKEQVFLVPTGEVFEQQT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | UHRF1 binding protein 1 |
| 别名 | BLT3A, BLTP3A, C6orf107, ICBP90 binding protein 1, UHRF1 binding protein 1 |
| 计算分子量 | 1440 aa, 159 kDa |
| 观测分子量 | 160-170 kDa |
| GenBank蛋白编号 | BC137120 |
| 基因名称 | UHRF1BP1 |
| Gene ID (NCBI) | 54887 |
| RRID | AB_3742108 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6BDS2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bridge-like lipid transfer protein family member 3A (BLTP3A), also known as UHRF1BP1, is a conserved protein characterized by six repeating beta-groove modules that form a hydrophobic groove for bulk lipid transfer between membranes. It localizes to VAMP7-positive vesicles and anchors them to LAMP1-positive lysosomes via its chorein domain and Rab7 interaction. Upon lysosomal membrane damage, BLTP3A rapidly dissociates and is re-recruited through its LIR motif binding to mATG8 proteins via the CASM pathway. This dynamic behavior suggests BLTP3A delivers lipids to aid in lysosomal repair (PMID: 40935891). Beyond its cellular function, BLTP3A has been identified as a genetic risk factor for systemic lupus erythematosus.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UHRF1BP1 antibody 24345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
